Figure 2.

Effects of S1P on coronary flow (CF) (A), left ventricular developed pressure (LVDevP) (B), +dLVP/dt_max (C) and −dLVP/dt_max (D) of isolated mouse hearts. S1P (10⁻⁶ M) or its vehicle was administered to the perfusate of isolated wild-type (WT) murine hearts for 5 min. The infusion was followed by a 20-min wash-out period. Administration of S1P resulted in a remarkable decrease in CF, which prevailed throughout the infusion and the wash-out period (p < 0.0001). CF reduction compromised left ventricular contractile performance as evidenced by a concomitant decrease in LVDevP, +dLVP/dt_max and −dLVP/dt_max (p < 0.0001). Mean ± SEM; n = 6, 9; #### p < 0.0001 vs. baseline (pre-infusion value), * p < 0.05 vs. vehicle; two-way repeated measurement ANOVA and Dunnett’s post hoc test.