Table 3.
Carbohydrate specificities of lectins employed in the lectin-based microarray and lectin blot. Lectins used in each method are marked with “+”. For the lectin-based microarray, the obtained S/N ratios are given in brackets.
| Lectin (Source) | Carbohydrate Specificity | Lectin Microarray | Lectin Blot |
|---|---|---|---|
| PNA (Arachis hypogaea) | Galβ1,3GalNAc | + (S/N < 3) | |
| MAL-I (Maackia amurensis) | NeuNAcα2,3Galβ1,4GlcNAc | + (S/N < 3) | |
| MAL-II (Maackia amurensis) | NeuNAcα2,3Galβ1,3(±NeuNAc2,6)GalNAc | + (S/N < 10) | + |
| PHA-L (Phaseolus vulgaris) | Tri/tetraantennary complex type N-glycans w/terminal Gal | + (S/N < 10) | + |
| DSL (Datura stramonium) | GlcNAcβ1,4GlcNAc oligomers; Galβ1,4GlcNAc | +(S/N < 10) | |
| GNL (Galanthus nivalis) | High mannose type N-glycans; Manα1,3Man | + (S/N < 10) | |
| GSL-I (Griffonia simplicifolia) | Galα1,3Gal; Galα1,3GalNAc | + (S/N < 50) | |
| WGA (Triticum vulgaris) | GlcNAcβ1,4GlcNAc; chitin oligomers; NeuAc | + (S/N < 50) | |
| SNA (Sambucus nigra) | NeuNAcα2,6Gal/GalNAc | + (S/N < 50) | + |
| NPL (Narcissus pseudonarcissus) | High mannose type N-glycans; Manα1,6Man | + (S/N < 50) | |
| LCA (Lens culinaris) | αDGlc, αDMan in N-glycans with Fuca1,6GlcNAc | + (S/N < 50) | + |
| PhoSL (Pholiota squarrosa) | Fucα1,6GlcNAc | + (S/N < 50) | |
| PHA-E (Phaseolus vulgaris) | Galβ1,4GlcNAcβ1,2Man with bisecting GlcNAc | + (S/N < 50) | + |
| AAL (Aleuria aurantia) | Fucα1,6GlcNAc; Fucα1,3(Galβ1,4)GlcNAc | + (S/N < 50) | + |
| ConA (Canavalia ensiformis) | Manα1,6(Manα1,3)Man | + (S/N > 50) | |
| RCA (Ricinus communis) | Galβ1,4GlcNAc | + (S/N > 50) |