Figure 6.

ATP-dependent remodelers and transcription. In wild type cells, Pol II levels are low at the promoter (promoter–proximal pausing is not generally observed in yeast), high on the gene body (elongating Pol II), and there is a peak of terminating Pol II downstream of the transcript termination site (TTS). In the isw1Δ chd1Δ double mutant, elongating Pol II levels are high relative to terminating Pol II levels, suggesting that elongation is inhibited and/or termination is facilitated. In the former case, close-packed di-nucleosomes may inhibit elongation; in addition, increased anti-sense cryptic transcription may result in inhibition due to Pol II collisions. In Rsc8-depleted cells, terminating Pol II levels are high relative to elongating Pol II, suggesting that termination is inhibited. Transcription levels are very low in these cells, suggesting an initiation defect also. Adapted from [77].