Figure 2.

The inhibitory effects of anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanins isolated from meoru, AIMs) and/or cis-diaminedichloroplatinum (CDDP) on MCF-7 cells human breast cancer cells. The cells were treated with 400 µg/mL of AIMs one hour before 5 µg/mL of CDDP treatment and incubated for 48 h. (A) After 48 h of treatment, pictures were taken representing cellular and nuclear morphology of the cells which showed damages cells and decreased cell proliferation. DAPI staining was used to show the deformed nucleus. (B) The inhibitory effects of combined AIMs and CDDP were analyzed through the trypan blue exclusion assay. “+” and “−” represents the presence and absence of the compound, absence of the compound specified. The data shown are mean ± SD of three different experiments performed separately. ** p < 0.05 versus treated group and *** p < 0.05 versus CDDP treated group.