Figure 3.

Synergistic effects between AIMs and CDDP on cell death of MCF-7 cells. MCF-7 cells were treated with 400 μg/mL of AIMs 1 h before 5 μg/mL of CDDP treatment and incubated for 48 h (A) Flow cytometry for cell cycle analysis. MCF-7 cells were stained with PI (Propidium Iodide) and subjected to flow cytometry analysis. The population of the cells in subG1 phase has been highly increased when AIMs and CDDP were treated together (B) flow cytometry with Annexin V/PI double staining. MCF-7 cells were treated with the given treatment (400 µg/mL of AIMs and 5 μg/mL of CDDP for 48 h) and stained with Annexin V/PI to analyze through flow cytometry. The number of cells in the fourth quadrant increased significantly showing high cell death when AIMs and CDDP treated together. “+” and “−“represents the presence and absence of the compound, absence of the compound specified.