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. 2020 Aug 9;25(16):3623. doi: 10.3390/molecules25163623

Figure 4.

Figure 4

The effect of CDDP with or without AIMs on NF-κB activity in MCF-7 cells. The proteins were isolated from MCF-7 cells treated with 400 µg/mL of AIMs and 5 µg/mL of CDDP for 48h. (A) Equal amounts of cell lysate (30 μg) were resolved by SDS polyacrylamide gels and transferred onto nitrocellulose membranes. The membranes were probed with the indicated antibodies and detected by the enhanced chemiluminescence detection system. Western blot analysis for Akt, NF-κB and NF-κB regulated proteins. The data shown here are representative of at least three independent experiments. (B) Densitometry analysis of Western blot bands. The values were normalized against actin and expressed as a mean of ± SD of at least three independent experiments * p < 0.05, ** p < 0.01, and *** p < 0.0001 between control and treated groups. “+” and “−” represents the presence and absence of the compound, absence of the compound specified.