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. 2020 Aug 9;25(16):3623. doi: 10.3390/molecules25163623

Figure 5.

Figure 5

The effect of combination treatment of TNF-α and CDDP on MCF-7 and MDA-MB-231 cells. (A) The cell viability is measured by the trypan blue exclusion assay. MCF-7 and MDA-MB-231 cells were pre-treated with 20 ng/mL of TNF-α 1 h at 37 °C followed by the treatment of 5 and 20 µg/mL of CDDP. The cells were stained with trypan blue and counted using a haemocytometer and percentage of viable cells were calculated. (B) The morphological representation of TNF-α treated MCF-7 and MDA-MB-231 cells with CDDP. The cells were pre-treated with 20 ng/mL of TNF-α 1 h at 37 °C followed by the treatment of 5 and 20 µg/mL of CDDP. The data shown are mean ± SD of three different experiments performed separately. ** p < 0.05 versus treated group and *** p < 0.05 versus CDDP treated group. “−” represents the absence of the compound, absence of the compound specified.