Table 1.

L. monocytogenes genes targeted by qPCR in this study.

Gene	Function and Scope of Use	Sequence (5′→3′)	Reference
IGS	Reference gene	F: GGCCTATAGCTCAGCTGGTTA	Rantsiou et al. 2012 [14]
		R: GCTGAGCTAAGGCCCCATAAA
		P: FAM-ATAAGAAATACAAATAATCATACCCTTTTAC-TAMRA
plcA	Escape from primary vacuoles	F: CTAGAAGCAGGAATACGGTACA	Rantsiou et al., 2012 [14]
		R: ATTGAGTAATCGTTTCTAAT
		P: HEX-AATTTATTTAAATGCATCACTTTCAGGT-TAMRA
hly	Lysis of vacuoles	F: CATGGCACCACCAGCATCT	Rodríguez-Lázaro et al., 2004 [20]
		R: ATCCGCGTGTTTCTTTTCGA
		P: HEX-CGCCTGCAAGTCCTAAGACGCCA-TAMRA
iap	Invasion associated secreted endopeptidase	F: AATCTTTAGCGCAACTTGGTTAA	Rodríguez-Lázaro et al., 2004 [20]
		R: CACCTTTGATGGACGTAATAATACTGTT
		P: HEX-CAACACCAGCGCCACTAGGACG-TAMRA
sigB	Transcription factor, regulation of virulence and stress-response genes	F: CCAAGAAAATGGCGATCAAGAC	Rantsiou et al., 2012 [14]
		R: CGTTGCATCATATCTTCTAATAGCT
		P: HEX-TGTTCATTACAAAAACCTAGTAGAGTCCAT-TAMRA

F, forward; R, reverse; P, probe; FAM/HEX, fluorochrome at 5′-end of the probe; and TAMRA, quencher of FAM/HEX at 3′-end of the probe.