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. 2020 Aug 18;11:1266. doi: 10.3389/fphar.2020.01266

Figure 3.

Figure 3

Effect of NCS 613 on p38 MAPK phosphorylation and NF-κB nuclear translocation upon TNFα stimulation. (A) TNFα increases and NCS 613 reduces p38 MAPK phosphorylation in A549 cells; (B) NCS 613 prevents NF-κB translocation into nucleus while TNFα stimulation greatly increases NF-κB p65-subunit translocation into nucleus. Shown are merged images (60x magnification) of DAPI staining and anti-mouse Alexa 654 recognizing primary p65-NF-κB antibody (n=3). Unpaired Student’s t-test. Mean ± SEM. **p < 0.01 and ***p < 0.001.