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. 2020 Aug 19;10:1610. doi: 10.3389/fonc.2020.01610

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Copyright © 2020 Huang, Huang, Zhao, Dong, Zhang, Fang, Sun, Kong and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison between the percentage of viable and dead cells under normoxia and hypoxia. Tumor cells in normoxic and hypoxic conditions were exposed to 4Gy physical dose photon, proton, or carbon-ion radiation. Cell survival was detected 48 h after irradiation using the Annexin V/7-AAD double staining kit. Representative flow cytometry images for each group are shown in the scatter plots. Statistical analysis of the cell survival and death percentages for each group are shown in the histograms. Each experiment was repeated at least three times. ***p < 0.001.