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. 2020 Aug 19;10:1563. doi: 10.3389/fonc.2020.01563

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Copyright © 2020 Gao, Qiao, Xing, Huang, Qian, Wang, Zhang, Zhang, Li, Cui and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-17-5p participates in stiffness-mediated effect on PTEN expression. (A) (i) The predicted binding sites of miR-17-5p in the PTEN mRNA 3′-UTR using the TargetScan database and the mutation sequences of PTEN mRNA 3′-UTR. (ii) Dual-luciferase reporter assay validated that miR-17-5p specifically binds 3′-UTR of PTEN. (B) (i,ii) PTEN expression is significantly decreased in HCC cells with miR-17-5p mimics (50 nmol/l) cultured on the 6-kPa stiffness substrate and increased in HCC cells with miR-17-5p inhibitor (50 nmol/l) under the 16-kPa stiffness substrate. (C) (i,ii) Higher matrix stiffness upregulates the expression of miR-17-5p, and knockdown of integrin β1 obviously reverses this change. Error bar represents SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.