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Complete setup of the non-linear microscope, which can be used for two-photon fluorescence and second-harmonic generation. The circled diagram shows the medium used to study the particles. The solvent containing the particles fills one of the cells of a multiwell support (Silicone Isolators™, GBL664206, Grace Bio-Labs) and a microscope slide is placed between the study volume and the microscope objective. Thus, turbulence due to evaporation of the liquid does not occur.
