Figure 3.

LAIR-1 triggering modulates monocytes activation upon in vitro stimulation. (A) PBMCs were pre-treated with anti-LAIR-1 agonist (Dx26) or isotype control (2 h) and stimulated overnight with TLR4 agonist- LPS or IFN-α and the expression of CD80 and HLA-DR was determined on gated monocytes, using flow cytometry. (B,C) MACS purified monocytes were pre-treated (2 h) with anti-LAIR-1 agonist (Dx26) or isotype control and stimulated 5 h with TLR4 agonist- LPS or IFN-α. (B) IL6, CXCL10, TNF, TLR7, IL8, IL10, CCL2, and STAT1 gene expression was evaluated by qRT-PCR and (C) protein production of IL-6, TNF-α, IL-8, and CXCL10 was measured by ELISA. Results are represented as paired samples. Statistically significant differences were considered when *p < 0.05 (Wilcoxon's test).