TABLE 2.
Antibiotic susceptibilities of bacteria before and after passaging.
| Bacterium | Antibiotics | MIC (mg/l) |
MBC (mg/l) |
||||
| P0 | P10 | X10 | P0 | P10 | X10 | ||
| S. aureus WIBG 1.2 | Vancomycin | 0.98 | 0.12 | 0.24 | 15.6 | 3.90 | 15.6 |
| Ciprofloxacin | 0.24 | 0.24 | 0.24 | 0.98 | 0.49 | 0.98 | |
| Erythromycin | 0.49 | 0.98 | 0.98 | 15.6 | 31.25 | 15.60 | |
| Fusidic acid | 0.49 | 0.49 | 0.49 | 1.95 | 3.90 | 3.90 | |
| Ampicillin | 2000 | 1000 | 1000 | ns | 2000 | 2000 | |
| Tetracycline | 0.98 | 0.45 | 0.49 | 7.81 | 3.90 | 3.90 | |
| S. aureus WIBG 1.6 | Vancomycin | 0.98 | 0.17 (0.06) | 0.49 | 0.98 | 1.95 | 1.95 |
| Ciprofloxacin | 1.95 | 0.49 | 1.95 | 15.60 | 3.90 | 15.60 | |
| Erythromycin | 31.25 | 15.6 | 15.60 | 62.50 | 62.50 | 62.50 | |
| Fusidic acid | 31.25 | 15.6 | 31.25 | 125 | 62.50 | 62.50 | |
| Ampicillin | 2000 | 1000 | 1000 | 2000 | 2000 | 2000 | |
| Tetracycline | 0.24 | 0.24 | 0.35 (0.133) | 1.95 | 1.95 | 1.95 | |
| MRSA | Ciprofloxacin | 1.95 | 1.95 | 1.95 | 3.90 | 3.90 | 3.90 |
| Fusidic acid | 0.12 | 0.12 | 0.12 | 0.98 | 1.95 | 1.95 | |
| Ampicillin | 2000 | 2000 | 2000 | ns | 2000 | ns | |
| Vancomycin | 0.98 | 0.11 | 0.24 | 3.90 | 1.95 | 1.95 | |
| S. epidermidis | Ciprofloxacin | 0.98 | 0.49 | 0.49 | 1.95 | 0.98 | 0.98 |
| Fusidic acid | 0.24 | 0.24 | 0.24 | 1.95 | 1.95 | 1.95 | |
| Vancomycin | 1.95 | 0.29 (0.12) | 0.98 | 15.6 | 3.90 | 7.81 | |
| Erythromycin | 0.49 | 15.6 | 0.49 | 1.95 | 15.60 | 3.90 | |
| Tetracycline | 7.81 | 62.50 | 15.60 | 15.60 | 62.50 | 31.25 | |
| S. pyogenes | Ciprofloxacin | 0.49 | 0.49 | 0.49 | 0.98 | 0.98 | 0.98 |
| Erythromycin | 0.24 | 0.98 | 0.35 (0.14) | 1.95 | 7.81 | 1.95 | |
| Tetracycline | 0.24 | 0.98 | 0.49 | 7.81 | 15.60 | 7.81 | |
| P. aeruginosa WIBG 1.3 | Ciprofloxacin | 0.29 (0.12) | 0.24 | 0.24 | 0.98 | 0.49 | 0.98 |
| Gentamicin | 0.98 | 3.90 | 1.95 | 1.95 | 7.81 | 1.95 | |
| Meropenem | 0.98 | 0.98 | 0.98 | 1.95 | 1.95 | 1.95 | |
| P. aeruginosa WIBG 2.2 | Ciprofloxacin | 0.03 | 0.24 | 0.03 | 0.24 | 0.98 | 0.24 |
| Gentamicin | 0.98 | 0.49 | 0.98 | 3.90 | 3.90 | 3.90 | |
| Meropenem | 0.98 | 0.49 | 0.49 | 1.95 | 0.98 | 1.95 | |
| E. coli WIBG 2.4 | Ciprofloxacin | 0.02 | 0.02 | 0.02 | 0.12 | 0.24 | 0.24 |
| Gentamicin | 0.98 | 3.90 | 1.95 | 3.90 | 15.60 | 3.90 | |
| Meropenem | 0.12 | 0.12 | 0.12 | 0.24 | 0.24 | 0.24 | |
Bold type indicates a ≥ 4-fold change when comparing baseline sensitivities (P0) to P10 and X10 values. Data are expressed as geometric means from biologically duplicated experiments, with each comprising technical triplicates. Standard deviations are given in the parentheses if the data varied between replicates. Non-susceptible (ns) denotes no sensitivity breakpoint determined as the value was in excess of the antimicrobial concentrations used in the broth dilution.