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. 2020 Aug 19;8:824. doi: 10.3389/fcell.2020.00824

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Copyright © 2020 Yu, Hu, Yang, Zhang, Song, Xiang, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of Tcf4 target genes in DPCs treated with Twist1, Tcf4, and siTwist1. (A–C) Western blot results of c-myc, survivin, and cyclinD1. (D–F) Quantitative analysis of (A–C). The expression levels of target genes were standardized to that of GAPDH. (G–I) qPCR results of c-myc, survivin, and cyclinD1, the expression level of target genes was standardized to that of GAPDH. N = 3. ^#P < 0.05, ^##P < 0.01 when compared with the Tcf4-treated group. *P < 0.05, **P < 0.01 when compared with the control group.