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. 2020 Aug 19;8:824. doi: 10.3389/fcell.2020.00824

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Copyright © 2020 Yu, Hu, Yang, Zhang, Song, Xiang, Yang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The interaction of Tcf4 and Twist1 in DPCs. (A,B) The expression of Tcf4 and Twist1 were detected by immunofluorescence in cultured DPCs. (A) Scale bar = 20 μm. (B) Scale bar = 5 μm. (C) The expression of Twist1 was determined by Western blot in nuclear extract and total extract from cultured DPCs. (D) The expression of Tcf4 and β-catenin in the anti-Twist1 precipitated complex in cultured DPCs. (E) The expression of Twist1 and β-catenin in the anti-Tcf4 precipitated complex in cultured DPCs.