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. 2020 Aug 17;10(8):1609. doi: 10.3390/nano10081609

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Schematic representation of different steps of the preparation of the 3D hybrid nanofiber aerogels and its structure. (B) Images of hematoxylin and eosin-stained (left) and Masson’s trichrome-stained (right) tissue slides at 4 and 8 weeks post-implantation into critical-sized calvarial defects in rats. (A1/A2, D1/D2) Unfiled defects; (B1/B2, E1/E2) the defects implanted by 3D hybrid nanofiber aerogels (PCG/BG = 60:40); and (C1/C2, F1/F2) the defects implanted by E7-BMP-2 peptide-loaded 3D hybrid nanofiber aerogel (PCG/BG = 60:40) (black arrow: blood vessel in aerogel; white arrow: aerogel residue; green arrow: new bone) (adapted from Weng et al. [249], with permission from John Wiley and Sons).