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. 2020 Aug 15;10(8):1607. doi: 10.3390/nano10081607

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Neuronal marker protein expressions in hNLCs cultured with Fe₃O₄NPs. Fe₃O₄NPs apparently affected the transdifferentiation of CL-hMSCs starting from 25 μg/mL: hNLCs at day 3 (a) and day 8 (b) of transdifferentiation exhibited a decrease of fluorescence intensity of neuron markers such as β-Tub III (green fluorescence), MAP-2 (green fluorescence) and NSE (red fluorescence). Black spots of Fe₃O₄NPs (intracellularly and on the cell membrane) were also visible. Nuclei were stained with Hoechst 33258. White arrows indicate Fe₃O₄NPs (dark spots). Scale bar: 100 μm.

Neuronal marker protein expressions in hNLCs cultured with Fe₃O₄NPs. Fe₃O₄NPs apparently affected the transdifferentiation of CL-hMSCs starting from 25 μg/mL: hNLCs at day 3 (a) and day 8 (b) of transdifferentiation exhibited a decrease of fluorescence intensity of neuron markers such as β-Tub III (green fluorescence), MAP-2 (green fluorescence) and NSE (red fluorescence). Black spots of Fe₃O₄NPs (intracellularly and on the cell membrane) were also visible. Nuclei were stained with Hoechst 33258. White arrows indicate Fe₃O₄NPs (dark spots). Scale bar: 100 μm.