Table 4.

Cell viability evaluation in hNLCs plus Fe₃O₄NPs (1–50 μg/mL) over time.

Fe3O4NP (μ/mL)
hNLCs	0	1	5	10	25	50
day 2	100 ± 4.20	99.52 ± 1.38	97.33 ± 2.18	98.97 ± 1.10	96.37 ± 1.42	90.79 ± 5.88 *
day 3	100 ± 6.58	98.46 ± 12.93	96.13 ± 7.08	94.39 ± 5.11	91.14 ± 2.15	90.75 ± 0.84 *
day 4	100 ± 3.76	101.39 ± 1.42	95.51 ± 2.07	94.45 ± 1.95	89.58 ± 0.66 *	89.56 ± 1.61 *
day 5	100 ± 2.41	92.90 ± 3.94	90.34 ± 4.35 *	90.11 ± 1.94 *	81.86 ± 2.51 *	81.19 ± 0.87 *
day 8	100 ± 4.48	92.92 ± 0.01	89.61 ± 4.04 *	84.01 ± 3.13 *	85.95 ± 2.56 *	78.64 ± 2.03 *

Data are expressed as percentage of viable cells (% of each control) and represent the mean ± S.D. * p < 0.05, statistical analysis by two-way ANOVA followed by Dunnett’s test.