Figure 4.

Hap4p and porin amount after induction of the Warburg effect in S. cerevisiae. The growth medium of S. cerevisiae was supplemented with 60, 120, and 180 mM of glucose or not at T0. (A) The relative levels of the co-activator Hap4p were assessed every 2 h. Total protein extracts were analyzed by SDS-PAGE and western blot using antibodies directed against Hap4p and phospho-glycerate kinase (PGK1) as a loading control. (B) Results representative of at least four experiments are shown. Hap4p signal was quantified, signal intensity was normalized to PGK1 and expressed in percentage of T0 for each experiment. The bar-graph shows mean % of T0 ± SD for all experiments. (C) The relative levels of a mitochondrial protein, Porin, were assessed every 2 h. Total protein extracts were analyzed by SDS-PAGE and western blot using antibodies directed against porin and phospho-glycerate kinase (PGK1) as a loading control. (D) Results representative of at least four experiments are shown. Porin signal was quantified, signal intensity was normalized to PGK1 signal and expressed in percentage of T0 for each experiment. The bar-graph shows mean % of T0 ± SD for all experiments.