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. 2020 Aug 19;10:1333. doi: 10.3389/fonc.2020.01333

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Copyright © 2020 Bouchez, Hammad, Cuvellier, Ransac, Rigoulet and Devin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Addition of glucose to a Crabtree-negative strain, Candida utilis. The growth medium was supplemented with 60 mM of glucose at T0 ( $▼$ ) or not ( $▲$ ). (A) Growth was followed for 8 hours. Results shown represent means of at least 15 separate experiments ± SD. (B) The respiratory rate was followed for 8 h. Results shown represent means of at least 15 separate experiments ± SD. (C) Glucose consumption (• C. utilis) or (x S. cerevisiae) and ethanol production ( $•$ C. utilis) or (x S. cerevisiae) were quantified. Results shown are means of at least four separate experiments ± SD. (D) Cytochrome content was quantified every 2 h after glucose addition to the medium. Results shown represent means of at least five separate experiments ± SD. (E) For each condition, growth was followed for 8 h in the presence or absence of Piericidin A (200 μM). Results shown represent means of at least three separate experiments ± SD.