Table 1.

Citrate synthase and cytochrome c oxidase activities in wild type and mutant cells.

		T0	T2		T4		T6		T8
		–	–	+	–	+	–	+	–	+
S. c – WT	CS	0.37 (± 0.2)	0.34 (± 0.19)	0.19 (± 0.1)**	0.30 (± 0.16)	0.08 (± 0.04)**	0.33 (± 0.17)	0.04 (± 0.01)**	0.33 (± 0.17)	0.04 (± 0.02)**
	COX	0.07 (± 0.02)	0.06 (± 0.02)	0.04 (± 0.01)	0.06 (± 0.02)	0.03 (± 0.01)**	0.05 (± 0.01)	0.02 (± 0.01)**	0.05 (± 0.02)	0.03 (± 0.01)*
S. c – Δhxk2	CS	0.39 (± 0.12)	0.48 (± 0.05)	0.28 (± 0.09)**	0.4 (± 0.14)	0.15 (± 0.05)***	0.44 (± 0.1)	0.1 (± 0.03)***	0.56 (± 0.13)	0.08 (± 0.03)***
	COX	0.09 (± 0.03)	0.09 (± 0.03)	0.06 (± 0.02)	0.08 (± 0.02)	0.05 (± 0.01)*	0.09 (± 0.03)	0.05 (± 0.02)*	0.1 (± 0.03)	0.05 (± 0.02)*
S. c – Δhap4↑	CS	0.28 (± 0.03)	0.35 (± 0.03)	0.23 (± 0.03)*	0.32 (± 0.07)	0.23 (± 0.02)	0.32 (± 0.04)	0.23 (± 0.05)*	0.35 (± 0.01)	0.22 (± 0.06)*
	COX	0.14 (± 0.04)	0.16 (± 0.04)	0.14 (± 0.03)	0.19 (± 0.04)	0.15 (± 0.02)	0.18 (± 0.02)	0.18 (± 0.05)	0.18 (± 0.02)*	0.14 (± 0.05)
S. c – Δhxk2hap4↑	CS	0.55 (± 0.06)	0.58 (± 0.09)	0.55 (± 0.06)*	0.51 (± 0.05)	0.38 (± 0.05)*	0.53 (± 0.05)	0.35 (± 0.08)**	0.57 (± 0.02)*	0.31 (± 0.05)*
	COX	0.1 (± 0.03)	0.1 (± 0.03)	0.1 (± 0.03)	0.11 (± 0.03)	0.1 (± 0.04)	0.13 (± 0.03)	0.11 (± 0.04)	0.13 (± 0.02)	0.12 (± 0.03)
C. u	CS	0.09 (± 0.04)	0.1 (± 0.04)	0.08 (± 0.03)	0.11 (± 0.05)	0.08 (± 0.04)	0.1 (± 0.03)	0.09 (± 0.02)	0.13 (± 0.04)	0.1 (± 0.02)
	COX	0.11 (± 0.03)	0.09 (± 0.03)	0.08 (± 0.03)	0.11 (± 0.03)	0.08 (± 0.01)	0.1 (± 0.04)	0.1 (± 0.03)	0.12 (± 0.04)	0.09 (± 0.04)

CS, citrate synthase; COX, cytochrome oxidase. Enzymatic activities were measured as stipulated in the Materials and Methods section and is expressed in μmol/min/mg protein. (–) indicates in the absence of glucose. (+) indicates in the presence of glucose. T0-2-4-6-8 are the hours of incubation w/ or w/o glucose. Results shown are means of at least three separate experiments ± SD.