Table 2.

Hexokinase activity in wild type and mutant cells.

	T0	T2		T4		T6		T8
	–	–	+	–	+	–	+	–	+
S. c – WT	UD	0.01 (± 0.02)	0.03 (± 0.03)	0.02 (± 0.03)	0.07 (± 0.05)	0.01 (± 0.02)	0.16 (± 0.04)*	0.01 (± 0.01)	0.21 (± 0.01)**
S. c – Δhxk2	UD	0.01 (± 0.01)	0.09 (± 0.09)	0.01 (± 0.03)	0.36 (± 0.14)**	0.01 (± 0.02)	0.4 (± 0.16)**	UD	0.53 (± 0.30)**
S. c – Δhap4↑	0.17 (± 0.07)	0.18 (± 0.09)*	0.18 (± 0.08)	0.29 (± 0.14)	0.44 (± 0.27)	0.3 (± 0.14)	0.45 (± 0.23)	0.32 (± 0.14)	0.38 (± 0.22)
S. c – Δhxk2hap4↑	0.01 (± 0.01)	UD	0.08 (± 0.06)	UD	0.09 (± 0.04)	UD	0.13 (± 0.08)	UD	0.12 (± 0.05)

Enzymatic activity was measured as stipulated in the Materials and Methods section and is expressed in μmol/min/mg protein. (–) indicates in absence of glucose. (+) indicates in the presence of glucose. T0-2-4-6-8 are the hours of incubation w/ or w/o glucose. Results shown are means of at least three separate experiments ± SD.

UD, Undetectable.