Table 1.
Cucumber leaf proteins responsive to salt and/or H2S treatment and identified by MALDI-TOF/TOF-MS.
| Spot no.a | Accession no.b | Protein name | Score | CI (%)c | Peptidesmatched | TMr (kDa)/TpId | EMr (kDa)/EpIe | SC(%)f | Species | T test p-value |
Change folds in intensityg |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Photosynthesis | |||||||||||
| 1(↑) | O65194 | Ribulose bisphosphate carboxylase small chain, chloroplastic | 65 | 82.41 | 11 | 20.238/8.86 | 96.846/4.47 | 50 | Medicago sativa | 0.049 | 6.41 |
| 6(↓) | XP_004144412.1 | Phosphoribulo kinase, chloroplastic | 574 | 100 | 22 | 46.497/5.97 | 41.667/5.40 | 66 | C. sativus | 0.009 | 0.52 ± 0.05 |
| 11(↑) | XP_004141091.2 | Carbonic anhydrase 2 isoform X2 | 75 | 99.85 | 2 | 35.702/6.19 | 30.455/6.02 | 7 | C. sativus | 0.009 | 2.21 ± 0.05 |
| 12(↓) | XP_004137418.1 | Ribulose-phosphate 3-epimerase, chloroplastic | 282 | 100 | 8 | 30.165/8.62 | 26.364/6.07 | 38 | C. sativus | 0.022 | 0.42 ± 0.06 |
| 13(↓) | ABN41481.1 | Putative chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit, partial | 267 | 100 | 7 | 10.907/5.24 | 16.582/6.31 | 54 | C. sativus | 0.035 | 0.23 ± 0.14 |
| 14(↓) | XP_004140080.1 | Chloroplast stem-loop binding protein of 41 kDa a, chloroplastic | 437 | 100 | 18 | 44.502/6.57 | 36.000/6.50 | 49 | C. sativus | 0.015 | 0.62 ± 0.01 |
| 17(↑) | XP_004140080.1 | Chloroplast stem-loop binding protein of 41 kDa a, chloroplastic | 57 | 90.58 | 10 | 44.502/6.57 | 35.667/6.70 | 24 | C. sativus | 0.017 | 12.34 |
| 30(↑) | XP_004142574.1 | Rubisco accumulation factor 1, chloroplastic | 116 | 100 | 14 | 49.424/4.98 | 44.500/4.79 | 29 | C. sativus | 0.016 | 2.14 ± 0.20 |
| 37(↑) | XP_011650288.1 | Ferredoxin–NADP reductase, leaf isozyme, chloroplastic isoform X2 | 735 | 100 | 20 | 40.299/8.54 | 32.364/6.56 | 57 | C. sativus | 0.041 | 1.96 ± 0.41 |
| 40(↑) | XP_004138462.1 | Ribulose bisphosphate carboxylase, chloroplastic | 425 | 100 | 8 | 51.768/5.58 | 44.487/4.82 | 26 | C. sativus | 0.042 | 2.29 ± 0.60 |
| 43(↑) | NP_001267658.1 | Sedoheptulose-1,7-bisphosphatase, chloroplastic-like | 673 | 100 | 26 | 42.075/5.96 | 37.564/4.93 | 62 | C. sativus | 0.048 | 1.79 ± 0.25 |
| 52(↑) | XP_004148579.1 | Chlorophyll a-b binding protein of LHCII type 1-like | 286 | 100 | 6 | 28.299/5.47 | 33.727/5.22 | 28 | C. sativus | 0.005 | 2.81 ± 0.02 |
| Protein folding, assembly and degradation | |||||||||||
| 3(↑) | XP_004149798.1 | Protein disulfide-isomerase | 670 | 100 | 35 | 57.046/4.88 | 65.084/4.89 | 68 | C. sativus | 0.046 | 1.91 ± 0.17 |
| 4(↑) | Q9SAD7 | Eukaryotic translation initiation factor 4B2 | 65 | 81.58 | 19 | 59.289/8.69 | 34.091/4.64 | 32 | Arabidopsis thaliana | 0.024 | 3.63 ± 0.89 |
| 5(↑) | XP_004139931.1 | 26S protease regulatory subunit 6B homolog | 409 | 100 | 19 | 46.911/5.42 | 55.712/5.57 | 47 | C. sativus | 0.033 | 1.75 ± 0.20 |
| 9(↑) | XP_004154254.1 | Probable carboxylesterase 13 | 132 | 100 | 8 | 35.086/5.70 | 37.500/5.79 | 28 | C. sativus | 0.014 | 5.47 ± 2.32 |
| 23(↑) | XP_004141139.1 | T-complex protein 1 subunit theta | 92 | 99.996 | 16 | 58.815/5.44 | 62.130/5.57 | 35 | C. sativus | 0.040 | 1.95 ± 0.28 |
| 39(↓) | XP_004141034.2 | Mitochondrial-processing peptidase subunit alpha | 77 | 99.892 | 8 | 54.059/5.62 | 54.328/5.50 | 16 | C. sativus | 0.031 | 0.63 ± 0.08 |
| 42(↑) | XP_004146811.1 | Eukaryotic initiation factor 4A-8 | 394 | 100 | 17 | 46.830/5.37 | 42.436/5.38 | 42 | C. sativus | 0.041 | 1.95 ± 0.22 |
| 48(↑) | XP_011654558.1 | Peptidyl-prolyl cis-trans isomerase A1 | 339 | 100 | 11 | 27.374/8.11 | 18.950/5.45 | 46 | C. sativus | 0.010 | 1.20 ± 0.03 |
| 55(↑) | XP_004141710.1 | Proteasome subunit alpha type-6 | 109 | 100 | 13 | 27.279/5.74 | 27.182/6.02 | 53 | C. sativus | 0.006 | 1.81 ± 0.02 |
| 61(↑) | XP_004141688.1 | Eukaryotic initiation factor 4A-11 | 138 | 100 | 8 | 46.797/5.38 | 44.590/5.37 | 22 | C. sativus | 0.011 | 1.11 ± 0.00 |
| Amino acid biosynthesis | |||||||||||
| 20(↑) | XP_011652060.1 | Elongation factor 1-delta isoform X1 | 274 | 100 | 9 | 24.670/4.45 | 31.087/4.48 | 46 | C. sativus | 0.019 | 1.64 ± 0.05 |
| 28(↓) | XP_004136850.1 | Ketol-acid reductoisomerase, chloroplastic | 348 | 100 | 15 | 63.533/6.22 | 63.486/5.82 | 30 | C. sativus | 0.031 | 0.64 ± 0.08 |
| 31(↓) | XP_004148484.1 | Acetylornithine deacetylase-like | 633 | 100 | 15 | 48.515/4.83 | 43.333/4.91 | 42 | C. sativus | 0.004 | 0.63 ± 0.02 |
| 32(↓) | XP_004148484.1 | Acetylornithine deacetylase-like | 748 | 100 | 18 | 48.515/4.8.3 | 42.333/4.86 | 50 | C. sativus | 0.050 | 0.44 ± 0.10 |
| 51(↑) | XP_004148484.1 | Acetylornithine deacetylase-like | 895 | 100 | 21 | 48.515/4.83 | 43.333/4.94 | 57 | C. sativus | 0.031 | 6.64 |
| 56(↑) | XP_004138495.1 | Cysteine synthase 1, mitochondrial | 142 | 100 | 14 | 40.323/7.71 | 34.091/6.55 | 40 | C. sativus | 0.005 | 1.56 ± 0.00 |
| Carbohydrate and energy metabolism | |||||||||||
| 7(↑) | XP_004145948.1 | Probable 6-phosphogluconolactonase 4, chloroplastic | 83 | 99.97 | 6 | 34.655/6.11 | 28.636/5.37 | 27 | C. sativus | 0.044 | 5.71 |
| 8(↑) | XP_004136700.2 | Secoisolariciresinol dehydrogenase-like | 202 | 100 | 8 | 29.089/5.72 | 29.545/5.63 | 32 | C. sativus | 0.015 | 3.78 ± 0.04 |
| 10(↑) | NP_001267500.1 | Alpha-galactosidase-like precursor | 131 | 100 | 9 | 45.668/5.60 | 49.686/5.96 | 34 | C. sativus | 0.007 | 2.33 ± 0.04 |
| 24(↑) | XP_004147146.1 | Malate dehydrogenase, cytoplasmic | 374 | 100 | 13 | 35.745/5.76 | 38.333/5.68 | 47 | C. sativus | 0.043 | 1.51 ± 0.18 |
| 25(↑) | YP_004849346.1 | ATPase subunit 1, mitochondrion | 901 | 100 | 22 | 54.959/5.58 | 53.650/5.74 | 50 | C. sativus | 0.009 | 1.34 ± 0.05 |
| 26(↑) | XP_004143301.1 | Enolase isoform X1 | 810 | 100 | 17 | 47.714/5.48 | 58.568/5.59 | 51 | C. sativus | 0.049 | 1.93 ± 0.34 |
| 27(↑) | XP_004149141.2 | Inositol-3-phosphate synthase-like | 525 | 100 | 22 | 56.342/5.49 | 60.264/5.77 | 45 | C. sativus | 0.014 | 1.16 ± 0.03 |
| 45(↑) | XP_011656794.1 | Pyruvate dehydrogenase E1 component subunit beta-1, mitochondrial | 455 | 100 | 11 | 39.627/5.60 | 33.551/5.28 | 40 | C. sativus | 0.033 | 1.47 ± 0.14 |
| 47(↓) | XP_004152516.1 | Glyceraldehyde-3-phosphate dehydrogenase, cytosolic-like | 878 | 100 | 19 | 36.604/6.25 | 34.275/6.56 | 76 | C. sativus | 0.007 | 0.75 ± 0.01 |
| 49(↑) | Q4VZG9 | ATP synthase epsilon chain, chloroplastic | 547 | 100 | 9 | 14.766/5.39 | 15.067/5.20 | 71 | C. sativus | 0.041 | 1.18 ± 0.01 |
| 60(↑) | BAJ23911.1 | Cytosolic alkenal/one oxidoreductase | 192 | 100 | 9 | 34.401/5.48 | 38.333/5.47 | 43 | C. sativus | 0.045 | 1.50 ± 0.12 |
| Secondary metabolite biosynthesis | |||||||||||
| 21(↑) | XP_004143844.1 | Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic/mito- chondrial |
123 | 100 | 11 | 42.779/5.50 | 32.536/4.85 | 35 | C. sativus | 0.046 | 1.48 ± 0.11 |
| 57(↓) | XP_004137828.1 | 29 kDa ribonucleoprotein, chloroplastic | 60 | 94.326 | 4 | 30.480/5.84 | 24.175/4.70 | 16 | C. sativus | 0.048 | 0.62 ± 0.06 |
| Stress and defense | |||||||||||
| 33(↑) | XP_004147353.1 | Actin-7 | 281 | 100 | 11 | 41.683/5.31 | 42.500/5.51 | 41 | C. sativus | 0.039 | 1.77 ± 0.23 |
| 36(↓) | XP_004142149.1 | Peroxidase 2 | 261 | 100 | 8 | 34.822/6.43 | 33.636/5.91 | 26 | C. sativus | 0.031 | 0.14 ± 0.14 |
| 41(↓) | AAA33129.1 | Peroxidase | 695 | 100 | 11 | 34.276/4.94 | 36.795/4.50 | 43 | C. sativus | 0.046 | 0.37 ± 0.10 |
| 46(↓) | XP_004147762.1 | Glutathione S-transferase U25-like | 352 | 100 | 13 | 25.057/5.18 | 24.010/5.10 | 38 | C. sativus | 0.035 | 0.71 ± 0.02 |
| Other and unknown proteins | |||||||||||
| 29(↓) | KGN63436.1 | Hypothetical protein Csa_1G000600 | 82 | 99.97 | 21 | 95.949/5.95 | 76.926/5.65 | 24 | C. sativus | 0.012 | 0.66 ± 0.02 |
| 38(↓) | XP_004136034.1 | Uncharacterized protein OsI_027940 | 65 | 98.44 | 7 | 22.557/4.45 | 24.780/4.44 | 37 | C. sativus | 0.043 | 0.59 ± 0.10 |
| 44(↓) | KGN54020.1 | Hypothetical protein Csa_4G268040 | 776 | 100 | 18 | 37.807/6.22 | 30.797/5.13 | 53 | C. sativus | 0.006 | 0.90 ± 0.01 |
| 58(↑) | KGN54259.1 | Hypothetical protein Csa_4G296130 | 634 | 100 | 12 | 35.144/8.98 | 27.029/5.75 | 41 | C. sativus | 0.014 | 1.24 ± 0.02 |
↑ and ↓: Up-regulated expression and Down-regulated expression, respectively.
aNumbering corresponds to the 2-DE in Figure S1 .
bAccession number from the NCBInr and SwissProt database.
cProtein score Confidence Interval.
dTMr and TpI are theoretical molecular mass and theoretical isoelectric point, respectively.
eEMr and EpI are experimental molecular mass and experimental isoelectric point, respectively.
fThe sequence coverage of identified proteins.
gData represents the mean of two independent replicates with standard error as indicated in Table S4 .