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. 2020 Aug 19;8:1024. doi: 10.3389/fbioe.2020.01024

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Copyright © 2020 Rasinkangas, Tytgat, Ritari, Reunanen, Salminen, Palva, Douillard and de Vos.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcriptomic analysis of PS24 compared to wild-type L. rhamnosus GG at OD_{600 nm} 2. (A) Volcano plot of differential expression compared for L. rhamnosus GG and PS24. Log2 fold change versus q-value is depicted. Data points marked in red have a log2 fold larger than 1 and a q-value smaller than 0.05. Positive fold change implies higher expression in the PS24 strain. (B) Mean normalized pili transcript count. Normalized expression of the spaCBA-srtC1gene cluster in the pilus-less PB12 derivative and PS24 derivative relative to wild-type L. rhamnosus GG. The housekeeping sortase A was included for comparison. (C) Top 20 upregulated genes in PS24 derivative. The top 20 genes with the highest log2fold value compared to wild-type L. rhamnosus GG at OD_{600 nm} 2. (D) Top 20 downregulated genes in PS24 derivative. The top 20 genes with the highest log2fold value in wild-type L. rhamnosus GG at OD_{600 nm} 2, compared to the PS24 derivative.