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Effect of eukaryotic initiation factor (eIF)3a on ionizing radiation (IR)-induced gamma-H2A histone family member X (γ-H2AX). Western blot (A) and immunofluorescence staining (B) analyses of γ-H2AX in H1299 cells with transient eIF3a knockdown and in NIH3T3 cells with stable eIF3a overexpression compared with their respective control cells following IR treatments.
