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. 2020 Sep 2;12:77. doi: 10.1186/s13073-020-00773-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — LINC00301 facilitates proliferation, migration, and invasion through regulation of EAF2/VHL/HIF1α pathway in NSCLC cells. a Represent images for EAF2, pVHL, HIF1α in LINC00301 KD, and LINC00301 OE A549 and SPC-A-1 cells analyzed by immunofluorescence. b IB tested EAF2, pVHL, HIF1α levels in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2 transfected A549, and SPC-A-1 cells. c IB tested EAF2, pVHL, HIF1α levels in pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 transfected in A549, and SPC-A-1 cells. d Colony formation assays (seeded in 6-well plate) tested cell proliferation in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. e Trypan blue staining tested cell viability in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. g Transwell migration assay tested migration activity in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. f Transwell migration assay tested migration activity in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. g Transwell invasion assay tested invasive activity in sh-NC, sh-1, sh-EAF2, sh-1 + sh-EAF2, pLenti-CMV-vector, pLenti-CMV-LINC00301, pLenti-CMV-EAF2 or pLenti-CMV-LINC00301 + pLenti-CMV-EAF2 transfected in A549 and SPC-A-1 cells. Assays were conducted in triplicate. *p < 0.05, means ± SD was shown. Statistical analysis was performed by Student’s t-test