Table 1.

Analysis of previously published bulk-segregant and lab-evolution WGS datasets using MutantHuntWGS

Birkeland et al. (2010)
SRA ID	SRR064545	SRR064546
Total Reads (% Mapped)	19,782,779 (92.80%)	20,015,390 (89.57%)	Additional Output Filtering
Variants Called in Control	10,022		Filtering by		Cutoff	Variant count (%)
Variants Called in Experimental		9,646	Variant quality score		>130	21 (0.21%)
Variants Unique to the Experimental strain with Variant Quality Scores > 100 (% of total)		188 (1.95%)	SnpEff Impact		>Moderate	55 (0.57%)
CDS Variants in SIFT output (% of total)		152 (1.58%)	Variant Quality Score + Impact		>130 + >Moderate	6 (0.06%)
Published mutation (s) Identified		Yes	SIFT		Deleterious	6 (0.06%)

Ottilie et al. (2017)
SRA ID	SRR3480136	SRR3490425	SRR3490399	SRR3490397	SRR3490304
Total Reads (Percent Mapped)	14,684,843 (93.61%)	7,935,729 (98.11%)	3,629,049 (97.53%)	5,611,439 (97.80%)	6,904,333 (97.91%)
Variants Called in Control	526
Variants Called in Experimental		367	298	336	377
Variants Unique to the Experimental strain with Variant Quality Scores > 100 (% of total)		11 (3.00%)	4 (1.34%)	7 (2.08%)	8 (2.12%)
CDS Variants in SIFT output (% of total)		4 (1.09%)	2 (0.67%)	4 (1.19%)	5 (1.33%)
Published Mutation(s) Identified		Yes	Yes	Yes	Yes

Goldgof et al. (2016)
SRA ID	SRR3480136	SRR3480251	SRR3480237	SRR3480212	SRR3480267
Total Reads (Percent Mapped)	14,684,843 (93.61%)	6,347,816 (94.49%)	7,480,005 (64.04%)	3,058,951 (98.11%)	2,545,805 (97.51%)
Variants Called in Control	526
Variants Called in Experimental		487	316	292	301
Variants Unique to the Experimental strain with Variant Quality Scores > 100 (% of total)		10 (2.05%)	8 (2.53%)	6 (2.05%)	4 (1.33%)
CDS Variants in SIFT output (% of total)		4 (0.82%)	3 (0.95%)	4 (1.37%)	2 (0.66%)
Published Mutation(s) Identified		Yes	Yes	Yes	Yes