Figure 2.

Mutation of Drs counteracts the TBI protective effect of mutation in Mtk. (A) The MI₂₄ of 1-7 day old mixed-sex flies containing mutations in both Mtk and Drs fed standard molasses food following TBI compared to the MI₂₄ of control flies and flies containing a mutation in either Mtk or Drs. The Mtk^R1 and Drs^R1 data are from Figure 1A. Each bar represents the average of ≥3 independent trials, and each dot represents the outcome for an individual vial of flies. Error bars represent the SEM. (B) Log₂ fold change in expression of Mtk and Drs between 1-7 day old uninjured Mtk^R1 and Mtk^R1;Drs^R1 whole male flies and control flies. All samples were normalized to expression of RpL32 and statistical testing was performed on ΔCt values (Pfaffl 2001). Each bar represents the average log₂ fold change in mRNA, and each dot represents the fold change for an individual sample. Error bars represent the SEM (n = 3). Significance was determined by a Student’s unpaired t-test. ***P < 0.001, ****P < 0.0001.