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. Author manuscript; available in PMC: 2020 Sep 2.
Published in final edited form as: J Neurooncol. 2020 Apr 27;148(2):231–244. doi: 10.1007/s11060-020-03517-5

Fig. 3. LB-100 and PD1 blockade result in a long-term antitumor antigen-specific memory of cured animals.

Fig. 3

(a) C57BL/6 CR and age-matched naïve control mice were re-challenged with 1.3×106 GL261 or B16 melanoma cells in the left striatum approximately 80 days after initial implantation. (b) Cumulative survival of mice over time. **P<0.01 (log-rank test). (c) CD8+ T cells collected from CR or naïve mice were exposed to either GL261 or B16 melanoma cells in the presence of α-CD28 for 24 hours. Golgi block was added after 20 hours. (d) Flow cytometry demonstrated enhanced IFN-gamma production by CD44+ CD8+ central memory T cells from cured mice when co-cultured with GL261 compared to B16. Memory T cells from cured mice had significantly enhanced IFN-gamma production when co-cultured with GL261 compared to memory T cells from naïve mice. (e) Representative FACS plots of IFN-gamma -γ+ CD8+ CD44+ T cells. Survival experiment was performed with 5 mice per group. CD8+ T cell data was obtained from the 5 surviving CR mice and 2 age-matched naïve controls. *P<0.05, **P<0.01 (one-way ANOVA with Tukey’s multiple comparison test)