Figure 1.

Total hemocyte counts for Manduca sexta larvae hemolymph collected 24 h after injection. Caterpillars were injected at fourth-instar head capsule or on day 2 of the fifth instar with either a constant amount of 10 μL of a Micrococcus lysodeikticus solution at a concentration of 10 mg/mL Manduca saline buffer or a mass-corrected concentration. Both age groups showed no statistically significant difference between constant and mass-specific bacterial injections (P > 0.05, two-way ANOVA; n = 3 for all treatment groups). Error bars indicate SEM.