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. Author manuscript; available in PMC: 2020 Sep 2.
Published in final edited form as: J Am Soc Mass Spectrom. 2020 Mar 23;31(5):1058–1065. doi: 10.1021/jasms.9b00107

Figure 3.

Figure 3.

Examples of neuropeptide image changes due to the wash step in serial sections of a crustacean brain. (a) An optical image of the crustacean brain, outlined in white. The elongated tubes point towards the commissural ganglion of the crustacean nervous system. (b)-(f) Crustacean neuropeptides that were removed due to the washing step. (g) A neuropeptide image example that did not have a change in intensity or localization due to the washing step. (h)-(m) Neuropeptides that had a clear signal increase due to the washing step. All washed-control comparisons are generated at the same TIC normalized signal intensity level (0 to (b) 1.82×10−3, (c) 2.40×10−3, (d) 3.00×10−3, (e) 2.07×10−3, (f) 1.15×10−3, (g) 2.38×10−3, (h) 1.96×10−3, (i) 2.55×10−3, (j) 1.27×10−3, (k) 2.05×10−3, (l) 1.18×10−3, (m) 3.34×10−3). The white line represents a 1 mm scale bar.