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. 2020 Aug 21;14(8):e0008609. doi: 10.1371/journal.pntd.0008609

Fig 3. Three W chromosome-specific genes are useful as sex biomarkers of S. japonicum in different geographical populations.

Fig 3

(A) Duplex PCR analysis of SjF4 and PSMD4. (B) Duplex PCR analysis of SjF6 and PSMD4. (C) Duplex PCR analysis of SjF9 and PSMD4. The upper bands correspond to W chromosome-specific gene amplifications and the lower bands correspond to PSMD4 amplifications. F1–3 represent biological triplicates of genomic DNA from adult females and M1–3 represent biological triplicates of genomic DNA from adult males. M represents a 50-bp DNA ladder marker.