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(A) Representative excerpts from the overlapped 1H–15N TROSY-HSQC spectra of loop residues of fl-FBD reconstituted in nanodiscs (red), fl-CYP2B4–fl-FBD with type-1 substrate benzphetamine (green). (B) Hotspot residues involved in CYP–FBD interactions obtained from NMR experiments.