Skip to main content
. 2020 Jun 5;15(7):1780–1787. doi: 10.1021/acschembio.0c00220

Figure 2.

Figure 2

Phylogeny and 8-oxogeranial reduction activities of representative ISY/PRISE throughout land plants. Phylognetic tree inference was performed with W-IQ-Tree (http://iqtree.cibiv.univie.ac.at/) using maximum-likelihood method and JTT+I+G4 substitution model. Branch support values were calculated using ultrafast bootstraping with 1000 replicates. A tree was visualized using FigTree (http://tree.bio.ed.ac.uk/software/figtree/). Bars represent catalytic efficiencies (s–1 M–1) of 8-oxogeranial reduction activity, compared to those of progesterone reduction (as percentage) for selected ISY/PRISEs. [Circles (●) and triangles (▲) indicate the occurrence of cardenolides and iriroids, respectively, in the species to which the sequences belong.]