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GC-MS analysis of activities of selected PRISEs. Total ion scan chromatograms are shown for each enzyme assayed with 8-oxogeranial (left), progesterone (middle), and testosterone (right). No activity on cholest-4-en-3-one was detected. [Legend: S, substrate; a–c, iridodials; d, nepetalactol; e, 5β-androstan-17β-ol-3-one (5β-dihydroprogesterone); and f, putative 5β-androstan-17β-ol-3-one (5β-dihydrotestosterone). Asterisk (*) denotes a detectable signal (see Figures S2 and S3).]
