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. 2020 Jun 5;15(7):1780–1787. doi: 10.1021/acschembio.0c00220

Table 1. Comparison of Activities on 8-Oxogeranial and Progesterone in Selected PRISE Homologues (Mean ± SD, n = 4).

  8-Oxogeranial
Progesterone
  KM (μM) kcat (s–1) kcat/KM (s–1 M–1) KM (μM) kcat (s–1) kcat/KM (s–1 M–1)
Coccomyxa subellipsoidea 7.0 ± 1.6 0.025 ± 0.001 3578.5 23.6 ± 5.8 1.611 ± 0.148 68 360.9
Physcomitrella patens 31.3 ± 8.6 0.066 ± 0.005 2103.2 9.5 ± 3.1 2.101 ± 0.204 220 392.3
Selaginella moellendorffii 1 4.6 ± 1.7 0.006 ± < 0.001 1273.0 11.7 ± 4.6 1.148 ± 0.143 98 119.7
Selaginella moellendorffii 2 n.d.     11.5 ± 3.0 1.832 ± 0.157 159 443.0
Selaginella moellendorffii 3 409.9 ± 74.2 2.029 ± 0.096 4950.0 8.5 ± 3.5 2.124 ± 0.254 249 032.7
Amborella trichopoda 2 n.d.     3.1 ± 0.8 1.412 ± 0.089 458 441.6
Amborella trichopoda 3 227.8 ± 29.7 0.321 ± 0.018 1410.0 4.5 ± 1.7 0.678 ± 0.067 149 958.8
Arabidopsis thaliana 1 41.8 ± 2.75 0.155 ± 0.004 3706.6 6.0 ± 2.5 1.865 ± 0.216 312 081.7
Arabidopsis thaliana 2 7.4 ± 3.0 0.002 ± < 0.001 308.1 5.7 ± 2.3 1.566 ± 0.148 275 026.3
Digitalis lanata 1 3421.0 ± 1102.0 0.064 ± 0.015 18.6 6.5 ± 2.0 1.230 ± 0.111 189 376.4
Digitalis purpurea 2 17.7 ± 5.9 6.317 ± 0.615 356 489.8 6.3 ± 1.9 1.881 ± 0.170 298 571.4
Catharanthus roseus 5 8.5 ± 1.5 5.835 ± 0.302 688 739.4 28.8 ± 11.5 2.094 ± 0.332 72 784.2