Table 1. Comparison of Activities on 8-Oxogeranial and Progesterone in Selected PRISE Homologues (Mean ± SD, n = 4).
8-Oxogeranial |
Progesterone |
|||||
---|---|---|---|---|---|---|
KM (μM) | kcat (s–1) | kcat/KM (s–1 M–1) | KM (μM) | kcat (s–1) | kcat/KM (s–1 M–1) | |
Coccomyxa subellipsoidea | 7.0 ± 1.6 | 0.025 ± 0.001 | 3578.5 | 23.6 ± 5.8 | 1.611 ± 0.148 | 68 360.9 |
Physcomitrella patens | 31.3 ± 8.6 | 0.066 ± 0.005 | 2103.2 | 9.5 ± 3.1 | 2.101 ± 0.204 | 220 392.3 |
Selaginella moellendorffii 1 | 4.6 ± 1.7 | 0.006 ± < 0.001 | 1273.0 | 11.7 ± 4.6 | 1.148 ± 0.143 | 98 119.7 |
Selaginella moellendorffii 2 | n.d. | 11.5 ± 3.0 | 1.832 ± 0.157 | 159 443.0 | ||
Selaginella moellendorffii 3 | 409.9 ± 74.2 | 2.029 ± 0.096 | 4950.0 | 8.5 ± 3.5 | 2.124 ± 0.254 | 249 032.7 |
Amborella trichopoda 2 | n.d. | 3.1 ± 0.8 | 1.412 ± 0.089 | 458 441.6 | ||
Amborella trichopoda 3 | 227.8 ± 29.7 | 0.321 ± 0.018 | 1410.0 | 4.5 ± 1.7 | 0.678 ± 0.067 | 149 958.8 |
Arabidopsis thaliana 1 | 41.8 ± 2.75 | 0.155 ± 0.004 | 3706.6 | 6.0 ± 2.5 | 1.865 ± 0.216 | 312 081.7 |
Arabidopsis thaliana 2 | 7.4 ± 3.0 | 0.002 ± < 0.001 | 308.1 | 5.7 ± 2.3 | 1.566 ± 0.148 | 275 026.3 |
Digitalis lanata 1 | 3421.0 ± 1102.0 | 0.064 ± 0.015 | 18.6 | 6.5 ± 2.0 | 1.230 ± 0.111 | 189 376.4 |
Digitalis purpurea 2 | 17.7 ± 5.9 | 6.317 ± 0.615 | 356 489.8 | 6.3 ± 1.9 | 1.881 ± 0.170 | 298 571.4 |
Catharanthus roseus 5 | 8.5 ± 1.5 | 5.835 ± 0.302 | 688 739.4 | 28.8 ± 11.5 | 2.094 ± 0.332 | 72 784.2 |