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. 2020 Jun 3;54(13):8094–8103. doi: 10.1021/acs.est.0c01751

Figure 1.

Figure 1

Experimentally determined Hg(Cys)2 concentration (± SD, n = 4) as a function of Geobacter membrane concentration in the competitive ligand exchange experiment. pH ∼4.0 and I = 10 mM NaClO4. Different concentrations of membranes (1–19 mg C L–1 corresponding to 0.4–7.1 μM Mem-RStot) were pre-equilibrated in 0.5 μM Hg(NO3)2 for 24 h followed by the addition of Cys to reach a final concentration of 2 μM. The concentration of Hg(Cys)2 was measured at equilibrium. Dashed lines demonstrate the modeled concentrations, optimized by R software using R package PHREEQC to minimize the merit-of-fit, Σ(model – experiment)2/Σexperiment2, at 2.5%.