Skip to main content
. 2020 Jun 4;142(26):11324–11342. doi: 10.1021/jacs.0c04924

Figure 4.

Figure 4

(A) Overlay of three X-ray crystal structures of E. coli DHFR with the Met20 loop crystallized in different conformations. Shown here are structures of DHFR with the Met20 loop in the closed (red, PDB: 1RX2), occluded (magenta, PDB: 1RX4), and open (cyan, PDB: 1RE7) conformations, based on data provided in conjunction with ref (107). (B) Schematic overview of the hydride transfer reaction catalyzed by DHFR, in which dihydrofolate is reduced to tetrahydrofolate, and NADPH is oxidized to NADP+.