Figure 7.

Overview of directed evolution experiments of the in silico designed retro-aldolase RA95, showing the increase in activity toward that of natural enzymes. Shown here are (A) the catalytic efficiencies of various evolved RA95 variants toward (R)-methodol, (B) the mutations introduced in each variant, and (C) the corresponding position of the different mutations on the structure, based on kinetic and structural data presented in refs (165 and 166). Reproduced with permission from ref (10). Copyright 2018 the authors, published by the Royal Society. All rights reserved.