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. 2020 Sep 2;6(36):eabb0333. doi: 10.1126/sciadv.abb0333

Fig. 5. Rap1 activates gene transcription without inducing histone exchange.

Fig. 5

(A and B) mRNA and H3HA incorporation levels at the Rap1-regulated RPS13 and RPL30 genes and at the inactive STE3 and active ADH1 control genes under normal conditions or following TBP anchor away. (C) Same but following auxin (IAA)–mediated degradation of Rap1 fused to auxin-inducible degron (AID; Rap1AID). See fig. S5 for experimental details. (D and E) Activation of CUP1 by the Ace1 AD and Rap1 AD. Cells depleted for endogenous Ace1 (see fig. S3D) were made to express the Ace1 DBD alone (none) or fused to either the Ace1 or Rap1 AD. Gene expression and H3HA incorporation were monitored, as before.