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. 2020 Sep 2;6(36):eaba3992. doi: 10.1126/sciadv.aba3992

Fig. 5. Centrosome dynamics defects after PC and AC3 KD.

Fig. 5

(A) Maximal distance between nucleus and centrosome during CK of neurons electroporated with centrin-RFP and GFP, CRE-GFP, or miRAC3-GFP in Kif3alox/lox background: The maximal distance was reduced in cilium-ablated and AC3 KD neurons [GFP 12.32 ± 0.42 μm versus CRE 10.42 ± 0.35 μm and miRAC3 10.84 ± 0.44 μm; one-way Kruskal-Wallis test (χ2 = 13.54, P = 0.003, df = 3), followed by Nemenyi test (*P < 0.05)]. (B) Percentage of CK efficiency in neurons electroporated with centrin-RFP and GFP, CRE-GFP, or miRAC3-GFP in Kif3alox/lox background: The percentage of inefficient CK was increased in cilium-ablated and AC3 KD neurons. (GFP 26% versus Cre 43% and miRAC3 38%, Pearson’s χ2 test = 10.75, P = 0.01). The black lines represent the median. GFP: N = 3, n = 50; CRE: N = 3, n = 46; miRAC3: N = 3, n = 61.