Table 2. Proximity ligation assay detects association of FBF-1 with CCR4-NOT complex component CCF-1.

PLA foci density was determined in maximal intensity projections of confocal image stacks encompassing germline SPC zones of the indicated strains. Reported values are mean ± SD derived from three independent biological replicates (3xflag::ccf-1; gfp::fbf-1 and 3xflag::ccf-1; gfp::fbf-2) or a single replicate (3xflag::ccf-1; gfp), all reared at 24°C. Differences in PLA density between 3xflag::ccf-1; gfp::fbf-1 or 3xflag::ccf-1; gfp::fbf-2 and the control 3xflag::ccf-1; gfp were analyzed by one-way ANOVA with Dunnett’s post-test. N, number of germline images analyzed.

Genotype	PLA density in SPC zone (/um^2) x 10−2	P value,vs. control	N
3xflag::ccf-1; gfp::fbf-1	5.2 ± 2.4	<0.0001	32
3xflag::ccf-1; gfp::fbf-2	1.1 ± 0.8	ns	27
3xflag::ccf-1; gfp	0.6 ± 0.2	n/a	12