Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | H1/WA01 embryonic stem cell line | WiCell | RRID:CVCL_9771 | Male |
| Cell line (Homo sapiens) | H28126 induced pluripotent stem cell line | Pollen et al., 2019 | Male | |
| Cell line (Homo sapiens) | 13234 induced pluripotent stem cell line | Bhaduri et al., 2020 | RRID:CVCL_0G84 | Female |
| Transfected construct (Homo sapiens) | CMV::GFP Adenovirus | Vector Biolabs, 1060 | 1:200 dilution | |
| Transfected construct (Aequorea victoria) | pCAG-EGFP | Subramanian et al., 2011 | Electroporation | |
| Transfected construct (Homo sapiens) | Scramble shRNA | Addgene plasmid # 1864 | RRID:Addgene_1864 | Electroporation (1 µg/ul) |
| Transfected construct (Homo sapiens) |
Raptor_1 shRNA | Addgene plasmid # 1857 | RRID:Addgene_1857 | Electroporation (1 µg/ul) |
| Transfected construct (Homo sapiens) | pLKO.1-TSC2 | Addgene plasmid # 15478 | RRID:Addgene_15478 | Electroporation (1 µg/ul) |
| Biological sample (Homo sapiens) | Primary Cortex Tissue Samples | UCSF Gamete, Embryo and Stem Cell Research Committee (GESCR) approval | GW16-19 | |
| Antibody | anti-Sox2 (Mouse monoclonal) | Santa Cruz | RRID:AB_10842165; Cat# sc-365823 |
(1:500) |
| Antibody | anti-Hopx (Mouse monoclonal) | Santa Cruz | RRID:AB_2687966; Cat# sc-398703 |
(1:250) |
| Antibody | anti-Cofilin (Mouse monoclonal) | Santa Cruz | RRID:AB_11150468; Cat# sc-376476 |
(1:100) |
| Antibody | anti-Cdc42 (Mouse monoclonal) | Santa Cruz | RRID:AB_627233; Cat#, sc-8401 |
(1:200) |
| Antibody | anti-Cux1 (Mouse monoclonal) | Abcam | RRID:AB_941209; Cat#AB54583 |
(1:500) |
| Antibody | anti-pVim (Mouse monoclonal) | MBL International | RRID:AB_592969; Cat# D095-3 |
(1:500) |
| Antibody | anti-pHistone H3 (Mouse monoclonal) | Abcam | RRID:AB_443110; Cat# ab14955 |
(1:500) |
| Antibody | anti-Cryab (Mouse monoclonal) | Abcam | RRID:AB_300400; Cat# ab13496 |
(1:500) |
| Antibody | anti-Hopx (Rabbit polyclonal) | Proteintech | RRID:AB_10693525; Cat# 11419–1-AP |
(1:500) |
| Antibody | anti-pS6 (Rabbit polyclonal) | Cell Signaling | RRID:AB_331679; Cat# 2211S |
(1:500) |
| Antibody | anti-p4EBP1 (Rabbit monoclonal) | Cell Signaling Technology | RRID:AB_560835; Cat# 2855S |
(1:500) |
| Antibody | anti-DEPDC5 (Rabbit polyclonal) | Sigma | RRID:AB_2682655; Cat# HPA054969 |
(1:500) |
| Antibody | anti-Arp2 (Rabbit polyclonal) | Proteintech | RRID:AB_2221854; Cat#10922–1-AP |
(1:100) |
| Antibody | anti-Cleaved Caspase-3 (Rabbit polyclonal) | Cell Signaling Technology | RRID:AB_2341188; Cat#9661S |
(1:200) |
| Antibody | anti-Ctip2 (Rat monoclonal) | Abcam | RRID:AB_2064130; Cat# ab18465 |
(1:500) |
| Antibody | anti-BrdU (Rat monoclonal) | Abcam | RRID:AB_305426; Cat# ab6326 |
(1:500) |
| Antibody | anti-Eomes (Sheep polyclonal) | R and D | RRID:AB_10569705; Cat# AF6166 |
(1:200) |
| Antibody | anti-Sox2 (Goat polyclonal) | Santa Cruz | RRID:AB_2286684; Cat# sc-17320 |
(1:250) |
| Antibody | anti-NeuN (Guinea pig polyclonal) | Millipore | RRID:AB_11205592; Cat# ABN90 |
(1:500) |
| Antibody | anti-Gfp (Chicken polyclonal) | Aves | RRID:AB_10000240; Cat#, GFP-1020 |
(1:500) |
| Commercial assay or kit | G-LISA Activation Assay | Cytoskeleton, Inc, | catalog# BK124, BK127, BK128 | |
| Peptide, recombinant protein | BDNF | Peprotech | Cat# 450–02 | 100 ng/mL |
| Chemical compound, drug | Rapamycin | Sigma | Cat# R8781-200UL | 250 nM |
| Chemical compound, drug | 3BD0 | Sigma | Cat# SML1687 | 1 µM |
| Chemical compound, drug | CN04 | Cytoskeleton inc, | Cat# CN04 | 500 ng/mL |
| Software, algorithm | Imaris | Oxford Instruments | Imaris, RRID:SCR_007370
V9.5 |