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. 2020 Aug 18;9:e58622. doi: 10.7554/eLife.58622

Figure 2. Dynamic states of ORC1 AAA+ and ORC2 WH domains.

3D classification ORCO2WH was performed with the density map low-pass filtered to 20 Å and an E-step resolution limit of 20 Å. Two 3D classes from ORCO2WH contained densities for ORC1 AAA+ domain. The ORC1 AAA+ domain was fit into these densities which revealed the domain approaching the ORC1·4 position (a) and the auto-inhibited position (b). (c-f) In the ORC core, the ORC2 WHD was observed in two conformations: an alternate state, called the inactive state (c, e), and the auto-inhibited state (f), similar to the DmORC2 WHD (PDB ID: 4xgc) (d). The ORCO2WH inactive state (e) was determined by focused classification of the ORCO2WH with a tight mask around ORC2 WH.

Figure 2.

Figure 2—figure supplement 1. The dynamic states of ORCO2WH and ORCO1AAA.

Figure 2—figure supplement 1.

3D classification of ORCO1AAA (a) and ORCO2WH (b) were performed with each map low-pass filtered to 20 Å and an E-step resolution limit of 20 Å. Two 3D classes from ORCO1AAA (left) illustrate dynamic movements of ORC2 WHD in place, while two 3D classes from the ORCO2WH (right) illustrate dynamic movements of ORC1 AAA+ approaching the ORC1·4 position and the auto-inhibited position.