Figure 3.

(A) Schematic design of a [3 × 3] constitutional dynamic network, CDN “Z”, undergoing hierarchical biocatalytically guided selection processes. (B) The schematic structures of the nine constituents comprising CDN “Z”. The hierarchical control of the selection processes is shown in part A: treatment of CDN “Z” with EcoRI that cleaves constituent EF′ results in the selection of the [2 × 2] CDN “Z_E”; interaction of the survived CDN “Z_E” with HindIII that cleaves GE′ leads to the selection of constituent FG′; subjecting CDN “Z” to HindIII cleaves GE′, resulting in the selection of the [2 × 2] CDN “Z_H”; treatment of the selected CDN “Z_H” with EcoRI leads to the cleavage of EF′ and to the selection of FG′. Panel I - The cleavage of a fluorophore/quencher (F_i/Q_i)-functionalized substrate by the Mg²⁺-ion-dependent DNAzyme reporter unit associated with the respective constituent. Panels II and III - Schematic cleavage of the sequence-specific domains associated with constituents EF′ and GE′ by the endonucleases EcoRI and HindIII, respectively.