Figure 4.

(A) Time-dependent fluorescence changes generated by the Mg²⁺-ion-dependent DNAzyme reporter units associated with the nine constituents and (B) concentrations of the constituents derived from the respective catalytic activities shown in part A, by using the appropriate calibration curves (Figures S9 and S10): (i) in CDN “Z”; (ii) in EcoRI-guided selection of CDN “Z_E”; (iii) in HindIII-stimulated selection of constituent FG′ from the survived CDN “Z_E”. The error bars in part B were derived from N = 3 experiments. (C) Schematic hierarchical selection of [3 × 3] CDN “Z” including EcoRI-induced selection of [2 × 2] CDN “Z_E” from CDN “Z” and the subsequent HindIII-guided selection of constituent FG′ from CDN “Z_E”.