Figure 5.

(A) Time-dependent fluorescence changes generated by the Mg²⁺-ion-dependent DNAzyme reporter units associated with the nine constituents and (B) concentrations of the constituents derived from the respective catalytic activities shown in part A, by applying the appropriate calibration curves (Figures S9 and S10): (i) in CDN “Z”; (ii) in HindIII-dictated selection of CDN “Z_H”; (iii) in EcoRI-induced selection of constituent FG′ from the survived CDN “Z_H”. The error bars in part B were derived from N = 3 experiments. (C) Schematic hierarchical selection of [3 × 3] CDN “Z” including HindIII-induced selection of [2 × 2] CDN “Z_H” from CDN “Z” and the subsequent EcoRI-guided selection of constituent FG′ from CDN “Z_H”.