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. 2020 Jun 15;14(7):9021–9031. doi: 10.1021/acsnano.0c04031

Figure 5.

Figure 5

Analysis of three endonucleases by the DNA tetrahedron module shown in Scheme 2. (A) Analysis of Nt.BbvCI. Panel I: fluorescence spectra of FAM (a) in the absence of added Nt.BbvCI, (b) upon the addition of Nt.BbvCI, 0.2 U μL–1, (c) upon addition of EcoRI, 0.2 U μL–1, (d) upon addition of HindIII, 0.2 U μL–1. Panel II: fluorescence changes of FAM upon the addition of variable concentrations of Nt.BbvCI. Panel III: derived calibration curve corresponding to the fluorescence changes of FAM at different concentrations of Nt.BbvCI. (B) Analysis of EcoRI. Panel I: fluorescence spectra of ROX (a) in the absence of added EcoRI, (b) upon the addition of EcoRI, 0.16 U μL–1, (c) upon addition of Nt.BbvCI, 0.16 U μL–1, (d) upon addition of HindIII, 0.16 U μL–1. Panel II: fluorescence changes of ROX upon the addition of variable concentrations of EcoRI. Panel III: derived calibration curve corresponding to the fluorescence changes of ROX at different concentrations of EcoRI. (C) Analysis of HindIII. Panel I: fluorescence spectra of Cy5 (a) in the absence of added HindIII, (b) upon the addition of HindIII, 2 U μL–1, (c) upon addition of Nt.BbvCI, 2 U μL–1, (d) upon addition of EcoRI, 2 U μL–1. Panel II: fluorescence changes of Cy5 upon the addition of variable concentrations of HindIII. Panel III: derived calibration curve corresponding to the fluorescence changes of Cy5 at different concentrations of HindIII.